那如注射液激活腹腔巨噬细胞的体内外抗L615白血病的作用

IN VITRO AND IN VIVO ANTI-L615LEUKEMIA EFFECT OF PERITONEAL MACROPHAGES ACTIVAIED BY NARU-INJECTION

用蒙药那如注射液激活小鼠腹腔渗出性巨噬细胞观察对Ｌ６１５白血病的抗瘤活性。结果表明：转输那如激活巨噬细胞的继承性免疫－化疗明显延长晚期Ｌ６１５白血病小鼠的平均存活时间（Ｐ＜０．０１）。早期转输那如激活巨噬细胞的免疫治疗，小鼠生存期延长不多，而晚期未见延长。体外实验观察，那如激活巨噬细胞的杀瘤治性具有剂量依赖性，且与ＩＬ－１、ＩＬ－２协同杀瘤作用更明显（ｐ＜０．０１）。那如激活巨噬细胞产生的细胞因子与巨噬细胞合用比单纯细胞因子的杀瘤活性更强（ｐ＜０．０１）。此外，还观察到那如激活巨噬细胞对正常细胞无损伤作用。本文提示，那如激活巨噬细胞的转输治疗是肿瘤免疫治疗中又一新的，有潜力的方法。

Antitumor activity of murine peritoneal exudative macrophages activated by the Mongolian drug Naru-injection against mouse leukemia(L615)was studied. The results showed that the average survival time of late stage L615 was prolonged significantly during the adoptive immunochemotherapy(AICT)by transferring activated macrophages. In groups treated by transferring activated macrophages the prolongationof murine survival time during the early stages of immunotherapy was not too much,the pronlgation was not found during the late stages. In vitro,it was found that the tumoricidal activity of activated macrophayes wasdose-dependent,and synergism of Naru-injection and IL-1 or IL-2 was able to augment the tumoricidalactivity. The cytokines produced by activated macrophages combined with the macrophages have more strongtumoricidal activity than that of single cytokies(P<0.01).Moreover,macrophages activated by Naru-injection had no damaging effect on mormal cells.The study suggests that transferring macrophages activatedby Naru-jnjection could be a new and potential adoptive immunotherapy protocol for the late stage L615.