糖醇乙酯衍生物制备方法的改进──气相色谱法分离木糖、核糖、阿拉伯糖、葡萄糖和甘露糖

An Improved Method for Preparation of Alditol Acetates──Seperation of Xylose、Ribose、Arabinose、Mannose and Glucose by Gas Chromatography

通过对单糖进行ＮａＢＨ＿４还原，用１－甲基咪唑作催化剂和乙酸酐反应生成醇糖乙酯，反应在室温１０ｍｉｎ即可完成，利用糖醇乙酯易挥发的特性，用ＯＶ－１７０１石英毛细管柱进行分离，得到了五个不同的单峰。

he analysis of monosaccharides by G C. with conventional method of preparing irimethylsi-lylated oxime resulted in unresolved peak.Therefore,the procedure is need to be modified to ob-tain clear seperation of monosaccharides.The present procedure is as follows. Monosaccharidesare reduced with sodium borohydride and the resulting alditols are acetylated using 1-methyl-imi-dazole as catalyst.Acetylation is complete in 10 min by this procedure.The alditol acetates arecompletely seperated by OV-1701 quartz glass capillary Gas chromatography due to their highvolatility.