鸡跗、膝、髋关节部皮肤初级传入纤维向脊髓的投射──HRP法

The locations and the Central Projections of the Primary Af-ferent Neurons of the Tarsal Skin,the Knee Skin and the HipJoint Skin in Chicken by HRP Histochemistry

将ＣＢ──ＨＲＰ分别注入鸡跗关节背外侧皮肤、膝关节外侧皮肤和髋关节背侧的皮肤内，逆行追踪其初级传入神经元的位置及初级传入纤维向脊髓的投射。结果：１．跗关节皮肤的初级传入神经元胞体位于同侧Ｔ＿６－Ｓ＿５脊神经节内，以Ｓ＿２节为最高峰，Ｌ＿２节为１较低的峰，两峰间为１低谷。２．膝关节外侧皮肤的初级传入神经元胞体位于同侧Ｔ＿４－Ｓ＿５脊神经节内，集中在Ｔ＿４－Ｌ＿４节内，高峰位于Ｌ＿３节。３．髋关节背侧皮肤的初级传入神经元胞体位于同侧Ｔ＿６－Ｓ＿４脊神经节内，集中在Ｔ＿５－Ｌ＿４节，高峰位于Ｌ＿１节。标记细胞均以中小型为主。４．附关节皮肤的初级传人终未连续地分布于Ｌ＿２－Ｓ＿３的５个脊髓节段。横断面观，见标记的终未密集地分布于Ⅱ层的背１／３部，Ⅲ层的腹１／２部内和Ⅳ层（Ｍａｒｔｉｎ氏划分法）。于１层发现少量。５．膝关节外侧皮肤的初级传人终末分布于Ｌ＿１－Ｌ＿２和Ｓ＿２－Ｓ＿３不连续的两个脊髓区间内。横断面观，标记的终末密集地分布于Ⅱ层的中１／３和Ⅲ层的中１／３部内。６．髋关节背侧皮肤的初级传入终末分布于Ｔ＿６－Ｌ＿２和Ｓ＿２－Ｓ＿４两个脊髓区间内。横断面观，见标记的终末密集地分布于Ⅱ层的腹１／３部内和Ⅲ层的背１／?

lnjection of CB-HRP(conjugates of horseradish peroxidase with cholera-gen B-chain)into the skin on rarsal,knee area and the dorsal part of the hip joint skinin 3-4 month old chicken,The results were as follovvs:1.The labeled primary neuronsof the tarsal skin appeared ipsilaterally in T6-S5 spinal ganglia in S2 ganglion.Theknee skin,the T4-S5,mostly in L3.The hip joint skin,the T6-S4 and mostly in Ll.2.The primary afferent fibers from the tarsal skin projected to the dorsal third part of thelamina Ⅱ and the ventral half part of the Lamina Ⅲ and almost all part of Lamina Ⅳ(Martins lan1ina system)of L2-L3 sigments of the spinal cord,The knee skin,the mid-dle part of the laina Ⅱ and lamina Ⅲ,and all the lamina Ⅳ of L2-L3 and S2-S3 sig-ments,The hip joint skin,the ventral third prat of the Lamima Ⅱ and the Dersal thirdpart of the Lamina Ⅲ and the Lamina Ⅳ of T6-12 and S2-S4 sigments. The presentresult suggest that the Martins lamina system be available one for the birds spinal cord.