免疫酶技术检查EHF患者外周血单个核细胞中病毒结构蛋白抗原的研究

DETECTION OF STRUCTURAL PROTEIN ANTIGENSIN THE PERIPHERAL BLODMONONUCLEAR CELLS OF EHF PATIENTS WITH IMMUNOENZYME ASSAY

２２例ＥＨＦ患者于不同病期外周血单个核细胞涂片，用已知抗病毒核蛋白的ＭｃＡｂＡ３５及抗膜蛋白的ＭｃＡｂ３Ｇ＿１进行免疫酶染色，以检查病毒结构蛋白抗原。３病日时抗原检出率为６３．１％，７病日时达１００％，１７病日时仅为１８．２％，与抗体应答规律一致，只是其检出比抗体检出约提早３ｄ，且核、膜蛋白抗原的检出率间差异无统计学意义，结合ＥＨＦ患者对病毒结构蛋白免疫应答的特点，可预测患者的转归。同时该法也可用于ＥＨＰ患者早期快速诊断，并值得推广应用于其它病毒性疾病的诊断中。

The peripheral blood mononuclear cells of 22 EHF patients in different stadia were smearedand investigated for the nucleocapsid protein(NP)and membrane protein(MP)antigens by immunoenzymeassay(IEA)with antl-NP McAb A_(35)and anti-MP McAb 3G_1 on the 3th day of the onset the structuralprotein antigens were positive in 63.1%of cases,on the 7th day reached 100%,on the 17th day dropped to18.2%,which was correspond with antibodies response but the structural protein antigens could be detectedahead of antibodies for 3 days. IEA can be used in the early speedy diagnosis of EHF patients and is worthextending to other viral diseases.