克山病心肌细胞色素氧化酶活性的电镜观察

Observation on the activity of cytochrome oxidase in myocardium of Keshan disease with electron microscope

本文收集6例克山病,1例扩张型心肌病和5例意外事故死亡病例(作对照)尸检材料,分别取左,右心室肌和横膈肌,作肌细胞的细胞色素氧化酶活性产物的电镜观察,对各组病例肌细胞酶活性变化进行对比分析。结果表明,对照组心肌细胞色素氧化酶活性变化不明显;克山病组横膈肌细胞色素氧化酶活性产物轻度脱失,心肌细胞色素氧化酶活性产物脱失明显。而扩张性心肌病心肌细胞色素氧化酶活性虽有降低,但不如克山病明显。并对各组病例肌细胞色素氧化酶活性变化的意义作简要的讨论。

In this study,comparative observation on the activity of cytochrome oxidasein myocardium of 6 autopsy cases with Keshan disease,5 cases with unexpecteddeath and 1 case with dilated cardiomyopathy was performed.The activity of cytochrome oxidase in the diaphragma of 4 cases with Keshan disease was observed withelectron microscope as well.The results showed that the product of activity ofcytochrome oxidase in myocardial mitochondria of cases with unexpected deathdisappeared not obviously,but the product of activity of cytochrome oxidase in diaph-ragma of Keshan disease disappeared slightly.The product of cytochromeoxidase in myocardium of Keshan disease disappeared most obviously.Althoughthe activity of cytochrome oxidase in myocardium of case with dilated cardiomyo-pathy was decreased,It was less decreased than that in Keshan disease.This observationssuggest that disturbance of function of oxidative phosphorylation in myocardiumof Keshan disease made insufficient energy to supply the myocardium,eventuallyit led to structural and functional changes of myocardium.