摘要
用事故死亡者脑组织中提纯的人脑肌酸激酶（ＣＫ－ＢＢ）对ＢＡＬＢ／Ｃ小鼠进行免疫，应用杂交瘤技术将免疫小鼠脾细胞分别与２种骨髓瘤细胞（ＮＳ－１，６５３）融合，筛选得到８株能稳定分泌ＩｇＭ抗体的杂交瘤细胞。对分泌抗体经酶联免疫电泳转移试验（Ｗｅｓｔ－Ｂｌｏｔ）进行了鉴定，其中４种抗体能特异地与ＣＫ－ＢＢ的ＳＤＳ－ＰＡＧＥ电泳带结合。提示这些单克隆抗体有可能应用于肌酸激酶的结构功能研究和临床检验中。
Spleen cells from BALB/C mice immunized with purified human brain creatine kinase(CK-BB) were fused separately with 653 and NS-1 myeloma cells.Eight hybridoma cell lines secreting McAb against human CK-BB were obtained after clonings.Their specifications were identified by enzyme-linked immunoelectrotransfer blot techniques(West-Blot).The McAbs were identified as mouse IgM.The McAbs can be applied to the clinical laboratory test of creatine kinase and research about CK-BB.
出处
《青岛医学院学报》
1995年第1期1-4,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
肌酸激酶
单克隆抗体
制备
鉴定
creatine kinase
antibodies
monoclonal
hybridomase
mice
inbred BALB C
