急性白血病钙素基因甲基化的PCR检测

DETECTION OF HYPERMETHYLATION OF CALCITONIN GENE IN ACUTE LEUKEMIA BY POLYMERASE CHAIN REACTION

应用多聚酶链反应（ＰＣＲ）技术检测了１０例急性淋巴细胞白血病（ＡＬＬ）和２２例急性髓细胞白血病（ＡＭＬ）降钙素（ＣＴ）基因的甲基化程度。结果表明，７例ＡＬＬ与１６例ＡＭＬ的ＣＴ基因都发生了高度甲基化。临床完全缓解的３例ＡＭＬ和２例ＡＬＬ中有１例ＡＬＬ为阳性，另有１例阴性的ＡＭＬ于１个月后转为阳性，随后出现临床复发。提示急性白血病ＣＴ基因高度甲基化的ＰＣＲ检测，对ＡＬＬ与ＡＭＬ微小残留病有很重要的诊断价值，特别为缺乏恒定肿瘤细胞标志的ＡＭＬ提供了一个新的分子基因标志。

Polymerase chain reaction(PCR) technique was used to detect the hypermethylation in the calcitonin(CT) gene.An abnormal increase in the number of 'CCGG' sites methylation in the 5 regions of CT gene occurred in the cell samples from 7 cases of ALL and 16 cases of AML;Of 5 cases(2 ALL and 3 AML) of clinical complete remisssion,one(ALL) showed a postitive pattern and another(AML),which showed a negative pattern at first,turned into a positive pattern a month later and soon relapsed. All these showed that the increased methylation of the CT gene might provide an important molecular marker for biologic events in human cell transformation or tumor progression.They might prove clinically useful in monitoring the gene markers of patients with lymphoid,acute myelogenous neoplasms or minimal residual diseases.This is especially important to AML because of its lack of an appropriate tumor cell indicator.