摘要
应用同位素３２Ｐ后标记方法，对腹腔注射染苯小鼠外周血白细胞ＤＮＡ加合物进行检测。结果表明，苯在体内可形成至少２种加合物，并经Ｄ５层析排除了游离同位素产生的假斑。提示该方法对苯毒作用及致癌机理研究和对职业人群早期生物学监测具有重要意义。
This paper reports toxicologic research on DNA adducts of benzene by using 32P-postlabeling technique. We found at least two kinds of benzene-DNA adducts formed in peripheral leukocytes of mice after I. P.administration. This method is very important for the biomonitoring of people exposed to benzene and for the study of toxicity of benzene and its carcinogenic mechanism.
出处
《青岛医学院学报》
1995年第3期184-187,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
苯
加合物
磷
放射性同位素
DNA
benzene
covalent binding
DNA adduct
phosphorus radioisotopes
isotopelabeling
