电导法配合Logistic方程确定燕麦冰冻半致死温度

Measurement of Freezing LT_(50) by Electrical Conductivity method in Associated with The Logistic Equation in oats

采用电导法对８个燕麦品种在不同低温下细胞膜透性改变情况进行研究，然后配合Ｌｏｇｉｓｔｉｃ方程求拐点值确定冰冻半致死温度（ＬＴ＿（５０））。试验结果：低温处理下，燕麦组织电解质透出率增加呈Ｓ形曲线增长，在－４℃～－１０℃之间随着温度的下降，燕麦组织电解质透出率有一急剧升高的敏感区域；燕麦品种ＬＴ＿（５０）在－３．３～－８．０之间；品种永久１２抗冻性最强，品种Ｖｉｃｔｏｒｙ其次，品种Ａｔｈａｂａｓｃａ抗冻性最差。

Electrical conductivity method was used to determine the changes of cell mem-brane permeability of 8 oat varieties after freezing treatments,and the inflectional points oflogistic epuations were taken as freezing lethal temperature of 50%(LT_(50)). The resultsshowed that the electrolyte leakage percentage of oat tissue increatqed following a“S” shape.There was a sensitive range between the temperature of -4℃～-10℃ in which the electrolyteleakage of the tissue increased quickly. The LT_(50) of all varieties were between -3.3～-8.0℃.The freezing tolerance was the highest for the variety of YG-12,higher for Victory and low-est for Athabasca.