四种嵩草属植物叶片酯酶和过氧化物酶同工酶分析

Isozyme Analysis of Esterase and Peroxidase in Leaves of Four Kobresia Species

采用聚丙烯酰胺凝胶垂直板电泳技术，对四种嵩草属植物－小嵩草（ｋｏｂｒｅｓｉａｐｙｇｍ－ａｅａ）、矮嵩草（Ｋ．ｈｕｍｉｌｉｓ）线叶嵩草（Ｋ．ｃａｐｉｌｌｉｆｏｌｉａ）和嵩草（Ｋ．ｂｅｌｌａｄｉｉ）的叶片酯酶和过氧化物酶同工酶进行分析。试验结果显示，四个种的酯酶同工酶泳动成两大区域，Ｒｆ值分别为０．３８～０．８６和０．０８，过氧化物酶同工酶泳动形成５个区域，Ｒｆ值分别为０．９７，０．６８～０．７７，０．５２～０．６３，０．２９～０．３７和０．０８。酶谱比较结果表明，嵩草和线叶嵩草亲缘关系较近，而二者同小嵩草、矮嵩草间，以及小嵩草同矮嵩草之间亲缘关系较远。

Leaf isozymes of esterase and peroxidase in four Kobresia species-K. Pyg-maea, K.humilis, K.capillifolia and K.belladii-were analysed by vertical polyacrylamidegel electrophoresis. The results showed that esterase isozymes of these four species migratedinto two regions with Rf value 0.38～0.86 and 0.08, and peroxidase isozymes migrated intofive regions with Rf value 0.97, 0.68～0.77, 0.52～0.63, 0.29～0.37 and 0.08 respectively.The preliminaty comparative results of the four zymograms indicated that a closer relationexisted between K.belladii and K.capillifolia and a farther relation among K.pygmaea, K.humilis and the other two.