摘要
采用聚丙烯酰胺凝胶垂直板电泳技术,对四种嵩草属植物-小嵩草(kobresiapygm-aea)、矮嵩草(K.humilis)线叶嵩草(K.capillifolia)和嵩草(K.belladii)的叶片酯酶和过氧化物酶同工酶进行分析。试验结果显示,四个种的酯酶同工酶泳动成两大区域,Rf值分别为0.38~0.86和0.08,过氧化物酶同工酶泳动形成5个区域,Rf值分别为0.97,0.68~0.77,0.52~0.63,0.29~0.37和0.08。酶谱比较结果表明,嵩草和线叶嵩草亲缘关系较近,而二者同小嵩草、矮嵩草间,以及小嵩草同矮嵩草之间亲缘关系较远。
Leaf isozymes of esterase and peroxidase in four Kobresia species-K. Pyg-maea, K.humilis, K.capillifolia and K.belladii-were analysed by vertical polyacrylamidegel electrophoresis. The results showed that esterase isozymes of these four species migratedinto two regions with Rf value 0.38~0.86 and 0.08, and peroxidase isozymes migrated intofive regions with Rf value 0.97, 0.68~0.77, 0.52~0.63, 0.29~0.37 and 0.08 respectively.The preliminaty comparative results of the four zymograms indicated that a closer relationexisted between K.belladii and K.capillifolia and a farther relation among K.pygmaea, K.humilis and the other two.
出处
《青海畜牧兽医杂志》
1995年第4期9-11,共3页
Chinese Qinghai Journal of Animal and Veterinary Sciences