湟中马血清酯酶多态性的研究

Investigation on Polymorphism of Serum Esterase in Huangzhong Horse

采用聚丙烯酰胺凝胶电泳法对青海省湟中县９１匹马血清酯酶的多态性进行了研究。结果发现：①湟中马的血清酯酶受ＥＳ￣Ｆ，ＥＳ￣Ｉ，ＥＳ￣Ｓ和ＥＳ￣Ｏ４个等位基因的控制，其基因频率分别为０．４４５０，０．５３３０，０．０１１０和０．０１１０；②在被检湟中马的ＥＳ位点上共发现ＥＳＦ，ＥＳＦＩ，ＥＳＩ，ＥＳＩＳ和ＥＳＯ五种表型，以ＥＳＦＩ为优势表型（４７．２５％）；③ＥＳ等位基因频率无性别差异；④血清酯酶位点的基因杂合度，有效等位基因数和基因均质度指数分别为０．５１７６，２．０７３和０．２２３６。

Huangzhong horses were used to investigate the polymorphism of serumesteraes by means of a polyacrylamide gel electrophoresis in Qinghai Province. The resultswere as followsL: (1) The serum esteraes in Huangzhong horse was controlled bv four allelesincluding ES￣F,ES￣I,ES￣S and ES￣O, and the frequencies of them were 0.4450, 0.5330, 0.0110 and0.0110, resp. (2) Five phenotype including ES F, ES FI, ES I, ES IS and ES O were found onthe locus of serum esterase in Huangzhong horse examined and the ES FI type was a prepon-derant phenotype (47.25%). (3) There was not a significant differenee between the allele fre-quencies of both sex-groups. (4) The gene heterozygosity, the number of effective allele andthe gene homogeneity index on the locus of serum esterase were 0.5176, 2.073 and 0.2236,resp.