摘要
介绍了载脂蛋白ＡⅠ的一种分离纯化方法。用离心法制备人血清高密度脂蛋白（ＨＤＬ），乙醇／乙醚（１＇３，Ｖ／Ｖ）脱脂，经ＳｅｐｈａｃｒｙｌＳ－２００凝胶层析及ＤＥＡＥ－５２离子交换梯度洗提得纯化的载脂蛋白（ＡｐｏＡＩ）．ＳＤＳ－聚丙烯酰胺凝胶电泳证明为单一条带。
The method of purificating ApoA Ⅰ is presented. Human serum HDL isprepred with supercentrifugation, which is degreased with alcohol/ether(3/1V/V),chro-matogrphyed with Sephacryl S-200 and DEAE-52 to obstain purified ApoA Ⅰ,which isverified to be unique belt by gel electrophosis.
出处
《曲阜师范大学学报（自然科学版）》
CAS
1995年第2期81-84,共4页
Journal of Qufu Normal University(Natural Science)
关键词
载脂蛋白
凝胶电泳
分离纯化
血清蛋白
ApoA Ⅰ,supercentrifugation,chromatography,gel electrophosis.
