原子吸收分光光度法测定植株中钙、镁的几种样品前处理方法的比较

Comparison of Several Sample Pretreatment Methods for Atomic Absorption Spectrophotometric Determination of Calcium and Magnesium in Plants

本文比较了原子吸收分光光度法测定植株中钙、镁的三种样品前处理方法：干友化法、１ｍｏｌ／ＬＨＣｌ振荡提取法（振荡法）和１ｍｏｌ／ＬＨＣｌ静止浸泡提取法（浸泡法）．结果表明，振荡法和浸泡法对钙的测定结果平均分别为干灰化法的１０１％和９２．０％，对镁的测定结果平均分别为１０３％和９７．６％．振荡法和浸泡法与干灰化法之间不存在显著性差异（α＝０．０５），它们之间有极显著的正相关性（相关系数在０．９９以上）．因此，在大批量样品的常规分析中，１ｍｏｌ／ＬＨＣｌ提取法可代替干灰化法．

A comparative study on three sample pretreatment methods, dry ashing method, 1 mol/L HCl shaking extraction method (shaking method) and 1 mol/L HCl stationary soaking cxtraction method (soaking method),for atomic absorption spectrophotometric determination of calcium and magnesium in plants has been carried out and the results show that,(1) the average percentages of plant calcium values by shaking method and soaking method are 101% and 92.0% of the value by dry ashing method respectively and those of plant magnesium values 103% and 97.6% respectively;(2) there are not significant differences among shaking method,soaking method and dry ashing method(α=0.05),and (3) there are very extremely significant positive correlations among the three methods (all of the correlation coefficients are over 0.99).Therefore,1 mol/L HCl extraction method can be used in routine analysis instead of dry ashing method for determination of calcium and magnesium in plants.