高效液相色谱法检测人肝组织中黄曲霉毒素

Determination of Aflatoxin B_1 in Human Live Tissues with High Performance Liquid Chromatography

以人肝癌组织为研究材料，丙酮、氯仿为主要抽提溶剂，建立了用高效液相色谱技术检测肝组织中黄曲霉毒素Ｂ＿１的方法。采用μ－Ｐｏｒａｓｉｌ色谱往，氯仿、环己烷、乙腈、异丙醇为流动相，荧光检测器检测，激发波长３６５ｎｍ，发射波长４３５ｎｍ。方法的平均回收率为９６．８５％，变异系数为４．５２％，最低检测限为０．０５ｎｇ。１０例肝癌组织中有７例检出５。４９～７３．７０ｎｇ／ｇ不等的黄曲霉毒素Ｂ＿１。方法快速、准确、灵敏度高。

rapid, reproducible,and sensitive method was developed for the determination of aflatoxin B_1(AF B_1)in human liver by high performance liquid chromatography using acetone and chloroform as main extractingsolvents,A μ-porasil column was used,with chloroform-cyclohexane-acetonitrile-2-propanol as mobilephase,and fluorescence detection with excitation wavelength set at 365nm and emission at 435nm. The aver-age recovery of AF B_1 from liver tissues was 96. 85%,and the coefficient of variation was 4.52%, and theminimum detectable amount was 0.02ng.Aflatoxin B_1 was detected in 7 of 10 liver samples from hepatocellu-lar carcinoma patients.