高效液相色谱法测定病人腹膜透析液中头孢唑啉的含量

Determination of Cefazolin in the Peritoneal Dialysateof Patients by High Performance Liquid Chromatography(HPLC)

高效液相色谱法测定病人腹膜透析液中头孢唑啉的含量赵玉兰，李楠，孙映和，张杰（中国人民解放军总医院中心仪器室北京１００８５３）１前言头孢唑啉钠是目前临床上常用的抗感染药，广泛用于腹膜透析病人的感染预防。但对其预防感染的实用价值和用药剂量还需进一步探讨。...

he objective of this study was to develop an HPLC method for the detection of the cefazolin concentra-tion in peritoneal dialysate of patients,Separation of the drug was performed on a YWG C_(18) column,using a0. 01mol/L sodium acetate buffer(pH 4.80):acetonitrile(85 :15)mobile phase and monitored at 254nm,Theaverage recovery of cefazolin was 96.69%±0. 62. Within-day and between-day precisions were better than3.4%and 6.0%,respectively.Our studies indicate that this is a simple,rapid,sensitive and reproducible method.