摘要
高效液相色谱法同时测定强化牛奶中的维生素Ａ和Ｄ＿２赵慧芬，李克杰，曹燕叔，郑建国（北京奶牛中心北京１０００８５）１前言维生素Ａ，Ｄ＿２（ＶＡ，ＶＤ＿２）是人体所必需的营养物质。强化ＶＡＤ消毒牛奶的出现，需要建立快速准确的分析方法。用高效液相色谱法（Ｈ...
reversed-phase high performance liquid chromatographic method for the simultaneous determination ofvitamin A and D_2 in fortified milk has been developed. A couple of serially connected columns with μ-Bonda-pak C_(18)(300 × 3. 9mm, 10μm)and Novapak C_(18)(150 × 3. 9mm, 4μm)and methanol/water(96/4)mobile phasewere used. Vitamin A and D_2 were detected at wavelengths of 325nm and 265nm, respectively. By usingsaponification procedure, the recoveries of vitamin A and D_2 were in the range of 90～110%, and the coeffi-cients of variation were lower than 8%.
出处
《色谱》
CAS
CSCD
北大核心
1995年第1期61-62,共2页
Chinese Journal of Chromatography
关键词
HPLC
牛奶
VA
VD
high performance liquid chromatography,vitamin A and D_2,fortified milk
