高效液相色谱法测定血浆中表阿霉素浓度

Determination of Pharmoruvicin in Plasma by High Performance Liquid Chromatography(HPLC)

报道了高效液相色谱法测定血浆中表阿霉素浓度的实验方法。采用μＢｏｎｄａｐａｋＣ＿（１８）色谱柱，正磷酸、甲醇、乙腈、异丙醇为流动相。荧光检测器检测，激发波长为４５０ｎｍ，发射波长为５３０ｎｍ。血浆经离心后，取血清，用甲醇沉淀蛋白。方法的平均相对回收率为９９．４％±４．６％，日内和日间平均ＲＳＤ小于５％和６％。最低检测限为３ｎｇ。本方法快速、准确，灵敏度高。

reversed-phase HPLC method is described for the determination of pharmoruvicin in human plasma Thepharmoruvicin was separated On a YWG-C_(18) column and fluorescence detection with excitation wavelength set at450nm and emission at 530nm. The mobile phase was consisted of orthophosphoric acid-methanol-acetonitrileand propan-2-ol.Afier being centrifuged,the protein was precipitated with methanol.The average recovery ofpharmoruvicin from plasma was 99.14%±4.6%, Intra and inter day precisions(plasma samples) were betterthan 5%and 6%, respectively.The lower limit of detection inplasma was 3ng for pharmoruvicin, This methodis simple,rapid sensitive and of excellent precision.