摘要
反相高效液相色谱法测定核苷酸代谢产物赵云,王凤君,尤忠义,余斌(重庆第二军区大学烧伤研究所630038)1前言黄嘌呤、次黄嘌呤、肌苷、腺苷是人体在能量代谢过程中高能核苷酸分解代谢的产物。在缺血、缺氧等病理过程中,由于三磷酸腺苷(ATP)合成障碍或消耗...
method for the determination of high energy nucleotide′s metabolites of rabbit′s heart tissue by RP-HPLC with UltrasphereTM-C18 column at 254nm was established.The CVs were 1.4~8.8%。 The lowest de-tection limits were 6.25ng.This method is sensitive,reliable and suitable for the determination of nucleotide and it′s metabolites。
出处
《色谱》
CAS
CSCD
北大核心
1995年第2期134-135,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱
核苷酸
代谢物
黄嘌呤
肌苷
腺苷
eversed-phase high performance liquid chromatography, xanthine, hypoxanthine,inosine,adenosine,nucleotide′s metabolies.rabbit heart tissue