一种新的分离三硝基甲苯及其代谢产物的高效液相色谱方法

A New Method for Separation of 2,4,6-Trinitrotoluene(TNT) and Its Metabolites by High Performance Liquid Chromatography(HPLC)

一种新的分离三硝基甲苯及其代谢产物的高效液相色谱方法姚明（中国预防医学科学院劳动卫生与职业病研究所北京１０００５０）１前言三硝基甲苯（ＴＮＴ）是一种常用的炸药，职业接触会导致肝损伤及白内障。为了搞清其中毒机制，需对其在体内外的代谢转化进行研究。ＴＮＴ...

n HPLC method is described for separation of TNT and its main reduction metabolites. Two columns(LC-C(18) and LC-CN)are connected in series and operated isocratically at 1.0mL/min with water-methanol-tetrahydrofuran(60+ 35+ 5 ). The baseline separation of 4-amino-2,6-dinitrotoluene(4A) and 2-amino-4,6-dinitrotoluene(2A) ,which are main metabolites of TNT,is obtained(Rs=2.1). All the TNT and itsmetabolites,including TNT,2,6-diamino-4-nitrotoluene(2,6-DA),2,4-diamino-6-nitrotoluene(2,4-DA),4-hydroxylamino-2, 6-Ainitrotoluene(4HA), 4A and 2A , can be separated from each other with this method.