牛蒡子中木脂素的薄层扫描测定

Thin-Layer Chromatography(TLC ) Scanning Determination of Lignans in Arctium Lappaol Linne

在高效硅胶薄层板上分离了牛蒡子中４种２，３－苄基丁内酯型木脂素──牛蒡甙元（Ｉ）、牛蒡素Ｂ（Ⅱ）、牛蒡酚Ａ（Ⅲ）、牛蒡酚Ｆ（Ⅳ）。流动相为二氯甲烷－乙醚－甲醇－己烷（４：１：０．４：１），室温放置，自然显色。薄层扫描法定量，各木脂素的线性范围在０．４５１～４．６０８μｇ，回收率为９９．９０％～１００．９０％，并用此法测定了不同产地牛蒡子中木脂素的含量。方法简便、快速，样品用量少。

simple,rapid,and accurate TLC method for the separation and deternunation of the lignans:arcti-genin(Ⅰ),neoarc B(Ⅱ),lappaol A(Ⅲ)and lappaol F(Ⅳ)in arctium lappaol linne is described. Dichloromethane-ethyl ether-methanol-hexane(4:1:0.4:1) was used as developing solvent on an HPTLCsilica plate. The plate was saturated with the solvent for 20 nun and then developed using ascending tech-nique,After being evaporated at room temperature,the spots were identified with a Shimadzu CS-930 TLCscanner. The contents of the four lignans were calculated by comparison with standards spotted on the sameplate.The calibration curves were linear in the range of 0.451～4.603μg for the four lignans and the recoveries(%)were 100.11, 100.59, 100.90 and 99. 90 respectively. The method has been applied to the analysis ofvarious samples and can be used for the quality control of arctium lappaol linne.