用免疫亲和柱分离、高效液相色谱法测定花生和玉米中黄曲霉毒素的研究

Determination of Aflatoxin B_1,B_2,G_1,G_2 in Peanut and Corn with Immunoaffinity Column Cleanup and High Performance Liquid Chromatography

建立了用免疫亲和柱分离净化、高效液相色谱法测定花生和玉米中黄曲霉毒素（ＡＦＴ）Ｂ＿１、Ｂ＿２、Ｇ＿１、Ｇ＿２的方法。采用６０％甲醇提取样品中的ＡＦＴ，氢氧化铁沉淀吸附除去样品液中大部分杂质，再经过亲和柱将ＡＦＴ截留，进一步与杂质分离，洗脱后，加三氟乙酸衍生化处理，反相高效液相色谱法分离定量。对样品中四种ＡＦＴ的最低可测下限浓度均为１μｇ／ｋｇ，样品加标５～１０μｇ／ｋｇＡＦＴ，回收率在７６．９％～１１３．６％范围内。

method for the determination of aflatoxin B_1,B_2,G_1,G_2 in peanut and corn with immunoaffinity column cleanup and high performance 1iquid chromatography was established.sample was e xtracted with 60%methanol and interfering materials were removed by ferric hydroxide adsorption. AFTs were retained onto im- munoaffinity column,eluted with methanol and derivatized with TFA,The detectable concentration of AFT in sample was 1μg/kg and the average recoveries of AFT spiked at 5～10μg/kg were in the range of 76.9%～113.6%。