利用高效弱阴离子交换色谱法纯化超氧化物歧化酶

Purification of Superoxide Dismutase by High Performance Weak Anion Exchange Chromatography

利用高软弱阴离子交换色谱法简化了超氧化物歧化酶（ＳＯＤ）的纯化手续。在丙酮沉淀后，仅用一步色谱分离就能使来自牛血的Ｃｕ，Ｚｎ－ＳＯＤ达到电泳纯，活性回收率为８６．４％，比活为７７１１Ｕ／ｍｇ，纯化倍数提高至５２倍。此外，详尽讨论了色谱分离的条件。

e procedure of purifying superoxide dismutase was simplified using high performance weak anion ex-change chromatography.In only one chromatographic step following the acetone precipitation,Cu,Zn-super-oxide dismutase from bovine erythrocytes was purified to electrophoretic homogeneity wit h a recovery of86.4%. The specific activity of the enzyme preparation was 7711U/mg,and its purity was increased upto to52-fold.The conditions of chromatographic separation were also discussed in detail.