反相高效液相色谱法测定药物“金水宝”中腺苷及腺嘌呤含量

The Determination of Adenosine and Adenine in the Drug“Jin Shui Bao” by Reversed-Phase High Performance Liquid Chromatography(RP-HPLC)

反相高效液相色谱法测定药物“金水宝”中腺苷及腺嘌呤含量李来生（南昌大学应用化学研究所南昌３３００４７）１前言补益药“金水宝”胶囊是国家中药一类新药，它具有补肾保肺、秘精益气之功能。其药效成分腺苷及腺嘌呤的测定一般沿用薄层色谱法［１］，该法回收率偏低，...

method for determining adenosine(A)and adenine(B)in the drug of Jin Shui Bao by RP-HPLC on μ- Bondapak C18 (3.9mmi.d. ×300mm) using 0. 01mol/L KH_2PO_4- methanol(85 :15,V/V,pH 5.7 )as themobile phase(1. 5mL/min )is described in this paper. The UV detector was set at 254nm. Aand B were ex-tracted with 0. 01%acetic acid Recover1es were 97. 18%～101.92%for A and 98.35%～101.19% for B. The method is simple,rapid and accurate.