小鼠免疫脾细胞与SP2／0细胞融合的杂交瘤细胞的超微结构研究

ULTRASTRUTURAL STUDIES ON THE HYBRIDOMA CELLS DERIVED FROM FUSION OF IMMUNIZED SPLEEN CELLS OF MOUSE AND SP2/0 MYELOMA CELLS

对ＳＰ２／０骨髓瘤细胞、免疫脾细胞和由二者融合而分泌抗水貂肠炎病毒（ＭｉｎｋＥｎｔｅｒｔｉｔｉｓＶｉｒｕｓ，ＭＥＶ）单克隆抗体的杂交瘤细胞超微结构进行了研究．经ＭＥＶ免疫的动物的脾细胞糙面内质网发达，细胞质与细胞核的比例增大．贴壁生长的ＳＰ２／０细胞的表面中央为微绒毛集中区，其余部分则遍布皱褶，细胞质中内质网不发达．由ＳＰ２／０细胞和免疫牌细胞融合形成的杂交瘤细胞，表面均匀分布有皱褶，无微绒毛，细胞在贴壁基部周围伸出裙状伪足，扩大了贴壁面积，使细胞贴壁较牢固．本实验还对ＳＰ２／０细胞和杂交瘤细胞的染色体做了计数，二者的平均数分别为６７和９７，在传代培养中染色体有丢失现象．

The hybridoma cell clones secreting monoclonal antibody against mink entertitis virus (MEV) were established. The ultrastructures of spleen cells of mouse immunized with MEV and SP2/0 myeloma cells and the hybridoma cells of fusion from these two cell types were studied. The results of this study indicated that the ultrastructural features of the spleen cells immunized with MEV were different from that of nonimmunized mice. Their cytoplasmic contents were much richer and the endoplasmic reticula were relatively developing than that of spleen cells of nonimmunized mice. Scanning electron microscopy revealed that there was an area concentrated with microvilli at the apical center of SP2/0 cell surface. The external features of hybridoma cells were different from that of both SP2/0 cells and spleen cells. A skirt-like lamellipoda extruded from the basic periphery of hybridoma cells along the bottle bottom. It is likely that this structure was involved with more tight adhesion of hybridoma cells to bottle bottom.