一种单子叶植物总DNA提取方法的改进及应用

A SIMPLE PROCEDURE OF DNA ISOLATION FROM MONOCOTYLEDONOUS PLANTS AND ITS APPLICATION

本文报导了一种可用于多种单子叶植物总ＤＮＡ提取的简易方法，其特点是迅速简易、成本低、产量高、纯度好，所得粗提总ＤＮＡ产量达３００μｇＤＮＡ／ｇ组织，经ＲＮＡ酶（ＲＮａｓｅＨ）处理后，电泳检测未发现残余ＲＮＡ。该方法可用于不同用途的单子叶植物总ＤＮＡ的提取与纯化，如用于花粉管导入法分子育种的基因源植物总ＤＮＡ的提取、对转基因植物进行ＤＮＡ分析等研究。

A procedure for plant DNA isolation is reported in this paper.The procedure issimple. time-saving.and could be used in most of the monocotyledonous plants includingrice. wheat, maize.and others. DNA with high quantity (≥300 μg DNA/g tissue) andquality could be easily obtained using this method. However. this method was not suitablefor DNA isolation from dicotyledonous plants.