摘要
木槿愈伤组织耐盐细胞系是在含NaCl的培养基上通过屡代选择产生的。在无盐培养基中进行几代继代培养后的愈伤组织,由其分化的芽以及脱分比增殖的愈伤组织均表现了耐盐性。木槿耐盐细胞由于对NaCl的反应比未经选择的细胞积累了更多的Na ̄+、K ̄+,而且细胞体积较小。
Salt-tolerant lines of Hibiscus syriacus L.callus were selected by continuousgrowth on media containing NaCl.Salt resistance was retaines after several subcultures of calluson salt-free medium and after shoot regeneration and micropropagation,Salt-tolerantH. syriacus cells accumulated more Na ̄+and K ̄+in response to Nacl than did unselected cells andwere smaller.
出处
《山东师范大学学报(自然科学版)》
CAS
1995年第2期197-199,共3页
Journal of Shandong Normal University(Natural Science)
关键词
愈伤组织
木槿
耐盐性
变异体
植株再生
Callus
Hibiscus syriacus L.
regeneration
salt-tolerance
selection