PCR检测HBVDNA对HBsAg无症状携带者传染性的监测

POLYMERASE CHAIN REACTION FOR DETECTION OF HBV DNA TO SURVEY THE INFECTION OF HBsAg ASYMPOTOMATIC CARRIERS

用多聚酶链反应和斑点杂交技术检测了５０例ＨＢｓＡｇ无症状携带者血清中ＨＢＶＤＮＡ，检出率分别为５０％（２５／５０）和５８％（２９／５０）。抗－ＨＢｃ、抗－ＨＢｅ和ＨＢｅＡｇ阳性者ＨＢＶＤＮＡ的检出率高于阴性者。单，ＮＨＢｓＡｇ阳性无症状携带者ＨＢＶＤＮＡ在血中呈不同程度地波动，其排毒情况无明显规律可循。

Fifty serum samples of HBsAg asympotomatic carriers were detected by polymerase chain reaction (PCR) and dot hybridization respectively. The results showed that the HBV DNA positive rate was 50% to 58%.Its positive rate was much higher in the serum positive for anti-HBc, anti-HBe and HBeAg than the negative.The appearance of serum HBV DNA was fluctuation in the single HBsAg asympotomatic carriers proved no regularity for the elimination of hepatitis B virus.