室旁核注入八肽胆囊收缩素对兔Oddi括约肌肌电的影响

EFFECT OF CHOLECYSTOKININ OCTAPEPTIDE MICROINJECTION INTO PARAVENTRICULAR NUCLEUS ON ELECTROMYOGRAPHIC ACTIVITY IN RABBITS ODDI’S SPHINCTER

向５６只雄性家兔下丘脑室旁核（ＰＶＮ）内注射微量胆囊收缩素（ＣＣＫ－８），观察对Ｏｄｄｉ括约肌肌电频率的影响。结果：１．向ＰＶＮ注入微量ＣＣＫ－８肌电频率明显增加（Ｐ＜０．０１）；２．向ＰＶＮ先注入ＣＣＫ拮抗剂丙谷胺（ＰＧＭ）后注入ＣＣＫ－８，肌电增加效应被阻断（Ｐ＞０．０５）；３．向ＰＶＮ注入谷氨酸钠（ＭＳＧ）引起肌电频率增加与注入ＣＣＫ－８效应相似（Ｐ＜０．０１）；４．肌电频率增加效应不受心得安影响。用酚妥拉明、阿托品及切断双侧颈迷走神经均可部分抑制其效应。提示下丘脑ＰＶＮ可能是参与控制Ｏｄｄｉ括约肌运动的中枢之一，传出神经可能是交感神经α受体和副交感神经。

The purpose of this study is to determine the changes of electromyographic (EMG) frequency of Oddi’s sphincter after the microinjection of CCK-8 into the paraventricular nucleus(PVN)of rabbits. The results were as follows: 1.There is a significant increase of EMG frequency after the microinjection of CCK-8 into PVN (P<0.01). 2.Proglumide (PGM, CCK antagonist)into PVN significantly blocked the increase of EMG frequency caused by CCK-8 into PVN (P> 0. 05). 3. There is an increase of EMG frequency after the injection of monosodium glutamate(MSG) into PVN a similar effect to the injection of CCK- 8 (P<0.01).4.The increase effect of EMG frequency is not influenced by the propranolol and there is some decompression effect with atropine, phentolamine or bilateral vagal nerves cut. The results suggested that Oddi’s sphincter may be excited by the injection of CCK-8 into PVN. The PVN in hypothalamas may probably be one the centre that takes part in controling the movement of Oddi’s sphincter, the efferent nerves may be the sympathetic and parasympathetic nerves.