DNA含量、AgNOR计数在食管癌研究中的应用

STUDIES ON DNA CONTENT AND ARGYROPHILIC NUCLEOLAR ORGANIZER REGIONS PROTEINS IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA

用自动化图像分析术及银染技术测定了５０例食管鳞状细胞癌的肿瘤细胞核ＤＮＡ含量及ＡｇＮＯＲ计数，结合临床资料，探讨二者与食管癌的临床病理、预后及相互关系。结果发现，肿瘤分化越差，ＤＮＡ含量和ＡｓＮＯＲ计数越高；淋巴结转移组ＡｇＮＯＲ计数明显高于无淋巴结转移组；肿瘤ＤＮＡ含量与ＡｓＮＯＲ计数呈正相关。此项研究对揭示食管鳞癌的恶性生物学行为、估计预后和指导治疗提供了较可靠的方法。

Nuclear DNA content. and AgNOR were determined in 50 patients with squamous cell carcinoma of the esophagus by automated image analysis and argyrophilic technique. The specimens examined were all archival paraffin-embedded tissues which were curatively resected.The result showed that the DNA index(DI)was found to correlate significantly with both histologic grading (P<0.01)3, lymph node metastasis (P<0.05) and the extent of wall penetration by the tumor (P<0.05).The 1-year, 3-year, and 5-year survival rate was better in diploidy group (100%, 86.7%, and 73.7% respectively) than in aneuploidy group (80.0%, 28. 6% and 22.8% respectively), with statistically significant difference (P<0.01). The AgNOR count had been found to have correlation with the lymph node metastasis and histologic grading. The higher the AgNOR count, the higher lymph node metastasis rate was(P<0.05),and the lower the histologic grading (P<0.0l), the worse the prognosis was. In the same time, there was very good correlation between the AgNOR count and DNA content (r=0.37627, P<0.01). It is suggested that the tumor DNA content and AgNOR count are important quantitative variables which can indicate the aggressive behavior of the esophageal cancer and predict prognosis.