鸭乙型肝炎病毒在天然宿主中的特异性体液及细胞免疫应答

STUDIES ON HUMORAL AND CELLULAR IMMUNE RESPONSES AGAINST DUCK HEPATITIS B VIRUS IN IMMUNIZED DUCKS

用灭活的鸭乙型肝炎病毒（ＤＨＢＶ）免疫健康成年鸭，观察鸭外周血淋巴细胞的增殖，同时用间接ＥＬＩＳＡ法检测免疫鸭血清中抗ＤＨＢｓ／ｐｒｅｓ和抗ＤＨＢｃ。结果发现，在２次免疫后，４只鸭均产生低滴度的抗ＤＨＢｓ／ｐｒｅｓ，抗ＤＨＢｃ仅在１只鸭中一过性地出现，４只鸭中３只于第三次免疫后出现对ＤＨＢｓ／ｐｒｅｓＡｇ的淋巴细胞增殖反应，２只出现对ＤＨＢｃＡｇ的增殖反应。表明鸭对ＤＨＢｓ／ｐｒｅｓＡｇ的免疫应答性校对ＤＨＢｃＡｇ强，动态观察细胞和体液免疫出现的时相，多数鸭先测及抗体，用抗ＤＨＢｓ／ｐｒｅｓ被动免疫幼鸭可部分阻断ＤＨＢＶ的实验感染。

In ducks immunized with inactivated DHBV, specific cell mediated immune responses were assayed with an antigen-driven lymphocyte proliferation test which the authors developed for the first time. Antibody responses to DHBs/presAg and DHBcAg were assayed with an indirect ELISA. All four immunized ducks devloped low titers of anti-DHBs/pres while anti-DHBc was detected only in one duck after repeated immunization. Three of four ducks showed DHBs/presAgspecific lymphoproliferative response after 2 nd booster, lymphocytes from two ducks responded to DHBcAg at the same time. In the followup Studies, antibody responses preceded cellular responses. Duck serum with anti-DHBs/pres activity partially protected ducklings against DHBV challenge when injected 3 days in advance.