隐孢子虫病的病原诊断

ETIOLOGICAL DIAGNOSIS OF CRYPTOSPORIDIOSIS

在南京四所医院收集腹泻患者粪便1014人份,进行隐孢子虫感染调查。每份粪便涂片后先用金胺-酚染色法过筛检查卵囊,发现阳性或可疑标本,再用改良抗酸和沙黄-美蓝染色法检查。结果,金胺-酚染色法阳性13例,用后两种染色法都找到卵囊;可疑者5例,后两种方法卵囊均为阴性。金胺-酚和改良抗酸染色法联用时,方法简便,效果好。卵囊少时,沙黄-美蓝染色法不易发现阳性。

l014 faecal specimens were collected from 4 hospitals in Nanjing to investigate the infection of Cryptosporidium. Each faecal specimen was smeared and screened by auramine-phenol staining method for oocyst, the positive and suspected specimens were identified by modified acid-fast staining method and safranin-methylene blue staining method. The result showed that 13 spccimens were positive by auramine-phenol staining method and confirmed by modified acid-fast staining method. 5 suspected specimens were negative as shown by the latter two methods.The best result could be obtained when auramine-phenol staining method was first used and folloWed by modified acid-fast staining method. This-technique is simple, sensitive and reliable. It is difficult to find oocysts by safranin methylene blue staining method when the oocysts are few (Figs. l-3).