子胞蚴连同钉螺组织切片间接免疫酶染法诊断日本血吸虫病的初步研究

A PRELIMINARY STUDY ON DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA BY INDIRECT IMMUNOPEROXIDASE ASSAY USING DAUGHTER SPOROCYST-CONTAINING ONCOMELANIA TISSUE SECTIONS

将子胞蚴连同钉螺组织行石蜡切片,初步探讨了幼虫抗原片间接免疫酶染法(IIPA-DS)在诊断日本血吸虫病中的价值.结果表明,此法对112例确诊血吸虫病患者的敏感性为94.6％,血清滴度范围自原液至1:160,几何平均滴度1:20。101例正常人血清及24例正常家兔血清均未出现假阳性反应,与24例并殖吸虫病患者血清无交叉反应。IIPA-DS与IHA、COP及ELISA在检出率上符合程度高,在反应强度上一致性好。认为IIPA-DS适合我国的实际情况,值得进一步研究。

Schistosoma japonicum daughter sporocysts in Oncomelania tissue sections were, for the first time, used as antigen in the diagnosis of schistosomiasis japonica by indirect immunoperoxidase assay (IIPA-DS). The most strong peroxidase reaction was localized on the tegument of the daughter sporocysts and cercariae, and at some parts of cercariaf parenchyma, when IIPA-DS was carried out. Of 112 sera from proven cases of schisto-Bomiasis japonica, 106 (94.6%) were positive, the range of titre dilution was from 1:1 to 1:160, the geometrie mean of titres was 1:20. IIPA-DS highly coincided with IHA, COP and ELJSA in both sensitivity and strength of reaction. Sera from 101 blood donors and 24 normal rabbits were all negative. No cross reaction with sera from 24 patients with paragonimiasis was observed.