抗子宫内膜抗体和子宫内膜异位症的关系

Antiendometrial Antibody and Endometriosis

介绍一种改良的子宫内膜抗原提取方法，经ＳＤＳ－ＰＡＧＥ及Ｗｅｓｔｅｒｎｂｌｏｔ分析证实，产物中存在多条蛋白区带，分子量分别为１８．４２，５４，７１，８２及９１ＫＤ，另有三条大于９４ＫＤ，其中４２、８２、９１ＫＤ及３条大于９４ＫＤ的区带可和抗子宫内膜抗体发生特异反应。并在此基础上建立了抗子宫内膜抗体的ＥＬＩＳＡ测定方法，可用于人体液抗子宫内膜抗体的检测。该法灵敏度高，特异性好，方法稳定，操作简便。临床应用表明，５６例内异症患者血清抗子宫内膜抗体（ＩｇＧ）阳性率为６６．１％，明显高于非内异症患者（其它妇科疾患）和正常人（Ｐ＜０．０５）。

A Modified isolation of endometrail antigens method from the human endometrium tissues has been developed. The procedures involve collagense digestion of the endometrial tissues and purification through Sephadex G-100. There are several bands in the product, include 18, 42, 54, 71, 82, 91 KD and three bands lager than 94 KD indentified by SDS-PAGE.It has also been proved by Western blot, the 42, 82, 91 KD and three bands lager than 94 KD can react specially with anti-endometrial antibodies.The ELISA method has been estabilished,and can be used for detecting anti-endomitrial antibodies in human body fluids. Serum specimens from patients with endometriosis (n=56) and patients with non-endometriosis and normal persons (n=18) were tested for the presence of anti-endometrial antibody. The serum levels of anti-endometrial antibodies and significantlly different between the patients with endometriosis (n=56)and non-endometriosis (n=75) as well as the normal group (n=18) (P<0.05).