汉防已甲素对肝细胞生长增殖的影响

The Effect of Trandrine on Growth and Proliferation of RBL Liver Cells

采用流式细胞技术观察不同浓度的汉防已甲素（Ｔｅｔ）对大鼠ＲＢＬ肝细胞生长增殖的影响。结果表明，随着Ｔｅｔ浓度的增加（１０～６０μｇ／ｍｌ），Ｇ２→Ｓ期的进程加速，且Ｓ期ＤＮＡ含量及Ｇ１和Ｇ２期蛋白质含量增加呈一定的量效关系。经加入外源性Ｃａ￣（２＋）及ＡＴＰ证实Ｔｅｔ促进肝细胞生长增殖的作用与阻断Ｃａ￣（２＋）内流无关。提示具有钙通道阻断作用的Ｔｅｔ可促进肝细胞生长增殖。

The effects of different doses of tetrandrine (Tet)on growth and proliferation of cultured RBL Iiver cells were observed by FCM. The results showed that Tet can accelerate G1→S phase: DNA contents of S phase and protein contents of G_1 and G_2 were increased with increasing doses of Tet (10～60μg/ml), there is relation between doses and effects.The results further demonstrated that these actions of Tet have no corresponce with obstruction Ca￣ (2+) influx by adding extra Ca￣(2+) and ATP. We conclude that Tet, Having Ca￣(2+) antagonist actions, can promote growth and proliferation of RBL Iiver cells.