57例小儿支原体肺炎PCR检测及其意义

Detection of Fifty-seven Pediatric Mycoplasma Pneumonia Cases by Polymerase Chain Reaction and It's Clinical Significance

对５７例小儿支原体肺炎鼻咽分泌物中肺炎支原体ＤＮＡ进行ＰＣＲ检测。采用快速制备ＤＮＡ模板的方法，简化ＰＣＲ实验过程，结果４２例阳性，阳性率７３．７％，而用支原体培养分离方法，阳性率为５５．３％（ｘ２检验Ｐ＜０．０５），相差显著。对其中１３例ＰＣＲ阳性患者经红霉素静脉滴注治疗１０～１４ｄ后，再经ＰＣＲ检测，８例转阴性，５例仍为阳性。因此该方法既保持了ＰＣＲ的敏感性和特异性，又简化过程，可用于常规检测肺炎支原体，有助于支原体肺炎的早期诊断、早期治疗及疗效评价。

Mycoplasma DNA in nasopharynx secretion of fifty-seven pediatric mycoplasma cases is detected by using PCR quick DNA sample preparation method and simple PCR processes. The result is positive in forty-two cases by using PCR method with positive rate of 73.7%, while positive rate is only 55.3% by using mycoplasma culture method (P<0.05). Thirteen patients with PCR positive results are treated by venoclysis Erythromycin for 10～14 days and mycoplasma DNA is detected by PCR again,among them eight cases turn negative, and five cases still remain positive. So this method is not only more sensitive and reliable than conventional culture techniques,but also simplifies PCR processes. It can be used as routine mycoplasma pneumonia test,and helps to give early diagnosis, early treatment and curative effect evaluation.