犬急性坏死性胰腺炎血液流变学变化及川芎嗪的影响

Hemorrheologic Alteration in Dogs with Acute Necrotizing Pancreatitis and the Antagonistic Action of Tetramethylpyrazine

采用胰管内逆行加压注射犬自身胆汁和胰蛋白酶混合物诱发犬急性坏死性胰腺炎模型。术后动态观察血液流变学各项指标变化及川芎嗪的影响。结果表明，在犬急性坏死性胰腺炎早期即有全血粘度、红细胞聚集指数、红细胞和血小板电泳率等异常改变，而川芎嗪治疗组上述指标改变与假手术组接近：病理学检查显示非治疗组胰间质微血管中可见微血栓形成，腺泡及脂肪细胞环死。川芎嗪治疗组鲜见。本文结果提示在犬急性坏死性胰腺炎早期异常改变的血液流变学为胰组织微循环障碍、微血栓形成、组织缺血环死等一系列改变的重要因素，川芎嗪可有效地阻止或改善这些变化。

The purpose of this study is to evaluate hemorrheologic alteration in dogs with acute necrotizing pancreatitis (ANP) and the possible action of Tetramethylprazine (TMP) on it.ANP models were made in 107 healthy mongreal dogs by retrograde ductal injection of a mixture of autologus bile and trypsin. Dogs were randomly assigned to three groups:sham operation, untreated, and TMP treated groups. Hemorrheologic parameters and serum amylase activity were measured at 1h, 6h, and 24h after induction of ANP. Results of this study showed that all hemorrheologic parameters were significantly elevated at the early stage of ANP, and increased further until 24h after induction of ANP. Microthrombus were observed in pancreatic interstitial tissue. After treated with TMP, all the above-mentioned changes were prevented.The results suggest that hemorrheologic alteration is one of the key factors for a series of changes in ANP such as microcirculatory disturbance, microthrombsis, and ischemia in pancreatic tissue.Antagonistic action of TMP on hemorrheologic disturbances might account for the mechanism of TMP in treating experimental ANP.