摘要
从人心肌提取肌红蛋白(myoglobin,Mb)抗原,制备抗Mb血清和单克隆抗体,在此基础上建立了检测血清Mb的ELISA和滴金免疫测定法(dotimmunogoldfiltrationassay,DIGFA)。ELISA法检测范围25~800ng/ml。批内CV<5%,批间CV<7%。健康男、女献血员各32例和74例老年人,血清Mb测定结果,参考值上限分别为55.40、50.67和65.75ng/ml。15例急性心肌梗塞(AMI)患者血清Mb含量均大于130ng/ml.滴金免疫测定法可在5min内完成检测,Mb含量>100ng/ml为阳性。7例AMI患者检测结果均为阳性。两种方法结合应用对AMI的早期诊断及疗效观察极有价值。
Human myoglobin(Mb)extracted from human cardiac muscles was used to raise polyclonal and monoclonal antibodies against Mb.On this basis ELISA and dot immunogold filtration assay(DIGFA)for the determination of serum Mb have been developed.For the Mb ELISA the range of determination was 25~800 ng/ml,and the within-and between-run CVs were below 5% and 7% respectively.Based on the results of determination of serum Mb level of 32 cases each of male and female blood donors and 74 cases of healthy old age individuals,the reference upper limit normal value were 55.40,50.67 and 65.75ng/ml respectively.The serum Mb level of 15 oasses of acute myocardial infarction (AMI)was all above 130ng/ml.DIGFA could be completed within 5 minuts.Serum Mb concentration higher than 100 ng/ml showed positive result.The tests of serum samples of 7 AMI patients were all positive.It could be concluded that the ELISA and DIGFA were very useful in early diagnosis and monitoring of AMI.
出处
《上海免疫学杂志》
CSCD
北大核心
1995年第4期226-229,共4页
Shanghai Journal of Immunology