SLE患者细胞粘附分子CD11a、CD18、CD54表达初探

Expression of Cell Adhension Molecules CD11a,CD18 and CD54 in Patients with Systemic Lupus Erythematosus

利用流式细胞术及免疫荧光双染色法，检测３５例系统性红斑狼疮（ＳＬＥ）患者外周血细胞粘附分子表型（ＣＤ１１ａ、ＣＤ１８、ＣＤ５４）及淋巴细胞表型（ＣＤ３、ＣＤ４、ＣＤ８、ＣＤ４５、ＲＡ、ＣＤ４５ＲＯ）。结果表明，ＳＬＥ活动组ＣＤ８＋细胞增高，ＣＤ４＋ＣＤ４５ＲＡ＋细胞减少；ＣＤ４＋细胞表面ＣＤ１１ａ、ＣＤ１８表达降低，后二者在ＣＤ８＋细胞上表达增高；ＣＤ５４在ＣＤ２０＋细胞上增高。进一步发现，ＣＤ８＋细胞的ＣＤ１８增高与ＣＤ４＋ＣＤ４５ＲＡ＋细胞减少呈负相关（Ｐ＜００５），而与ＣＤ２０＋细胞表面ＣＤ５４增高呈正相关（Ｐ＜００１）。本文提示，细胞粘附分子可能在ＳＬＥ发病机理中占有重要意义。

The phenotypes of adhesion molecules(CDlla, CD18 and CD54)as well as the lymphocyte phenotypes (CD3,CD4,CD8,CD20,CD45RA and CD45RO)in the pheripheral blood cells of 35 patients with systemic lupus erythematosus(SLE)were determined by flow cytometric analysis and dual immunofluorescence.The experimental results indicated that,in active case the numbers of CD8+ cells increased and those of the CD4+CD45RA+ cells decreased.The expression of CDlla/CD18 on CD8+cells elevated but that on the CD4+ cells declined.The elevation of CD18 on CD8 cell inversely correlated with the declination of CD18 on CD4+ CD45RA+ cells,but positively correlated with the elevation of CD54 on CD20+ cells.The present study suggested that cell adhension molecules might be an important factors in the pathogenesis of SLE.