摘要
本文用钙离子荧光探针Ｆｌｕｏ－３和ＰＥ－ＣＤ３双标记的方法，通过流式细胞计测定ＰＷＭ对ＣＤ３表达量不同的胎儿胸腺细胞内游离钙浓度的影响。结果表明，ＰＷＭ能迅速诱导胸腺细胞并且主要是ＣＤ３＋细胞钙动员，７０ｍｉｎ后胞内钙基本降至基线水平；Ｈ７、ＣＰＺ和ＥＧＴＡ均能抑制ＰＷＭ诱导的钙动员。
Effects of PWM(pokeweed mitogen) on the calcium mobilization of human fetal thymocytes was measured by dual-color analysis using Fluo-3 and phycoerythrin(PE) -conjugated cD3 with nowcytometry.The results showed that: PWM induced a rapid calcium mobilization in thymocytes,mainly in CD3+ cells,and which could be inhibited by H7,CPZ and EGTA.
出处
《上海免疫学杂志》
CSCD
北大核心
1995年第6期348-351,共4页
Shanghai Journal of Immunology
