抗CD3单抗和rIL－2共刺激法诱生扩增的人CD3AK的免疫生物学特征

Biological Features and Phenotypes of Human Anti-CD3 McAb Activated Killer cells(CD3AK) Induced by Anti-CD3 McAb and rIL-2 Costimulation

抗ＣＤ３单抗和ｒＩＬ－２共刺激法诱生扩增的人ＣＤ３ＡＫ是异质性细胞群，其细胞表型以ＣＤ３＋Ｔ淋巴细胞和ＣＤ５６＋ＮＫ细胞表型为主，具有活化的淋巴母细胞形态学特征，常形成集落，并表达活化淋巴细胞的表面标记，如ＩＬ－２受体和ＭＨＣⅡ类抗原。

Human CD3AK induced by anti-CD3; McAb and rIL-2 costimulation were heterogeneous cell populations, their main phenotypes were those of T lymphocytes (CD3+ CD4+or CD8+), the next was CD56+ which was mainly expressed on NK-like cells. Morphologically, CD3AK were similar to those of lymphoblasts. Some of them expressed IL-2 receptor and MHC-II antigens characteristic for the activated T lymphocytes.