甲胎蛋自对H－22腹水肝癌小鼠脾淋巴细胞增殖的影响

Effect of Alpha-fetoprotein (AFP) on Splenocyte Proliferation of Mice Bearing Ascites Hepatoma-22 in Vitro

用抗甲胎蛋白（ａｎｔｉ－ＡＦＰ）的单克隆抗体亲和层析柱从脐带血中提纯ＡＦＰ。通过ＭＴＴ法体外观察人ＡＦＰ对Ｈ－２２腹水肝癌小鼠脾淋巴细胞增殖的影响。结果表明，Ｈ－２２小鼠脾淋巴细胞经不同浓度ＡＦＰ（１２．５－１００μｇ／ｍｌ）处理后，对刀豆素Ａ（ＣｏｎＡ）诱导的增殖反应明显减弱，抑制率达３９．８％－６６％，并有一定的浓度依赖关系；而用不同浓度的人血清白蛋白（ＨＳＡ，３．１～１００μｇ／ｍｌ）体外处理，则无明显抑制作用。另外，我们用反证法证实，ＡＦＰ抗体（５５、１１０μｇ／ｍｌ）能明显阻断人ＡＦＰ（５０、１００μｇ／ｍｌ）对Ｈ－２２小鼠脾淋巴细胞增殖的抑制作用。

In the present work,human alpha-fetoprotein (AFP) was purified from umbilical cord blood by means of anti-AFP monoclonal antibody (McAb) affinity chromatography and the effect of AFP on splenocyte proliferation of mice bearing ascites hepatoma-22(H-22) was studied by MTT method in vitro. The results showed that AFP (12.5, 100μg/ml) could suppress mouse splenocyte proliferation induced by concanavalin A (Con A, 5μg/ml) with a concentration-dependent relationship, the percent of inhibition was 39.8%-66%. Human serum albumin (HSA,3.1-100μg/ml) had no significant influence on the splenocyte proliferaton.Our experiments also demonstrated that the immunosuppression of AFP (50, 100μg/ml)could be significantly abolished by AFP antibody (55,110μg/ml).