脐血抗—HPCA—2和Tük3标记的CD34阳性细胞的流式细胞仪比较分析

COMPARATIVE FLOW CYTOMETRIC ANALYSIS OF CORD BLOOD CD34 POSITIVE CELLS LABELLED BY ANTI-HPCA-2-FITC AND TüK3

在标记脐血造血祖细胞表面抗原（ＨＰＣＡ），ＣＤ３４中，比较抗－ＨＰＣＡ－２－ＦＩＴＣ和Ｔｋ３（纯抗体）标记的ＣＤ（３４＋）细胞在流式细胞仪分析中的荧光特征及两种单抗标记的ＣＤ（３４＋）细胞与体外培养的粒单细胞集落形成单位（ＣＦＵ－ＧＭ），红系爆发形成单位（ＢＦＵ－Ｅ），和混合集落形成单位（ＣＦＵ－Ｍｉｘ）的相关性。结果发现脐血有核细胞中，抗ＨＰＣＡ－２阳性细胞占１．０５±０．７２％（ｎ＝１３），Ｔｋ３阳性细胞占２．０６±１．２５％（ｎ＝８），差别显著（Ｐ＜０．０５）。每毫升脐血两种抗体标记的细胞分别为９６．５６±５６．６４和２３１．４０±１６３．９３（Ｐ＜０．０５）。尽管ＨＰＣＡ－２阳性细胞与Ｔｋ３阳性细胞数量呈显著正相关（ｒ＝０．８７５，Ｐ＜０．０１），前者与ＣＦＵ－ＧＭ，ＢＦＵＥ，ＣＦＵ－Ｍｉｘ及集落总数ＣＦＵｓ均呈正相关，而后者仅与ＣＦＵ－ＧＭ，ＣＦＵｓ相关。研究提示在检测造血祖细胞时，用抗－ＨＰＣＡ－２－ＦＩＴＣ代替Ｔｋ３可降低假阳性，获得较好的ＯＤ（３４＋）细胞与ＣＦＵ间的线性关系。

When measuring cord blood CD34+ cells,flurescent features of antiHPCA2-FITC or Tk3 labelled(anti HPCA-2+ or Tuk3+) cells analysed by flow cytometry,and the relationships of CD34+ cells with CFU-GM, BFU-E,CFU-MiX were compared.The results showed that in nucleated cells, antiHPCA-2+ Cells were 1.05±0.72%(n=13), significantly lower than Tk3+cells,2.06l±1 .25%(n=8)(P<0.05).The absolute connts ofantiHPCA-2+cells versus Tk3+cells per microliter were 96.56 ±56.64 % vs 231.40±163.93(P<0. 05).Althongh the number of antiHPCA-2+ cells positively correlated with that of Tk3+cella (r=0.875,P<0.01),the former correlated with the numbers of CFU-GM,BFUE,CFU-Mix,the total number of CFU (CFUs),the later only with the numbers of CFU-GM,OFUs respectively. Onr stndy snggested that using antiHPCA-2-FITC instead of Tk3 might decrease falsepositivity and obtain better linear relationships of CD34+ cells with CFU.