震颤性麻痹动物模型的研究

An Experimental Study on Paralysis Agitans Model in Rat

选用ＳＤ雌性大鼠４７只，用６－羟基多巴胺（６－ＯＨＤＡ）注入右侧中脑被盖腹侧黑质内侧端，损毁黑质。分别于术后２、４、６和８周用阿朴吗啡（０．５ｍｇ／ｋｇ）诱发旋转，平均转速大于７圈／ｍｉｎ，并经生理心理学行为测试合格者为成功模型。结果，模型成功率达４８．９４％（２３只）。从成功模型中选取动物，在术后２周、１个月、２个月作灌注固定，制作脑组织标本漂片、冰冻切片及振动切片。发现酪氨酸羟化酶（ＴＨ）阳性细胞在存活２周大鼠约８５％以上消失，在存活１个月及２个月以上大鼠的黑质致密带，９８％左右ＴＨ阳性细胞消失，用ＴＨ加尼氏双色法：见右侧黑质细胞减少９５％左右，胶质细胞大量增生，对侧均正常。结果证实６－０ＨＤＡ选择性破坏ＤＡ、ＮＡ神经元。

orty-seven female Sprague-Dawley rats received stereotaxic injections of 6-OHDA (8μg/4μl)containing 0.02% ascorbic acid into the right medial substantia nigra of ventral tegmen mesencephalon.Twenty-three successful models were obtained by injections of 0.5 mg/kg apomorphine at 2,4, 6, 8 weeks after operations respectively. The rats,choosed from successful models, were poured and fixed,then floating slices, frozen slices and vibration slices of the brain were made separately 2weeks,1 month and 2 months after operation. The specimen were examined by ABC and revealed that the Tyrosine hydroxylase (TH) positive cells were decreased to about 15% in surviving 2 weeks rats. About 98% TH positive cells of compact substantia nigra were disappeared in surviving 1 month and 2 months rats.Right substantia nigra cells were found to be reduced to 5% with massive proliferation of glias by TH and nissl double technique, while the left substantia nigra areas were remain normal.This study confirms that dopaminergic and noradrenaline neurons were selectively destroyed by 6-OHDA.