摘要
选用SD雌性大鼠47只,用6-羟基多巴胺(6-OHDA)注入右侧中脑被盖腹侧黑质内侧端,损毁黑质。分别于术后2、4、6和8周用阿朴吗啡(0.5mg/kg)诱发旋转,平均转速大于7圈/min,并经生理心理学行为测试合格者为成功模型。结果,模型成功率达48.94%(23只)。从成功模型中选取动物,在术后2周、1个月、2个月作灌注固定,制作脑组织标本漂片、冰冻切片及振动切片。发现酪氨酸羟化酶(TH)阳性细胞在存活2周大鼠约85%以上消失,在存活1个月及2个月以上大鼠的黑质致密带,98%左右TH阳性细胞消失,用TH加尼氏双色法:见右侧黑质细胞减少95%左右,胶质细胞大量增生,对侧均正常。结果证实6-0HDA选择性破坏DA、NA神经元。
orty-seven female Sprague-Dawley rats received stereotaxic injections of 6-OHDA (8μg/4μl)containing 0.02% ascorbic acid into the right medial substantia nigra of ventral tegmen mesencephalon.Twenty-three successful models were obtained by injections of 0.5 mg/kg apomorphine at 2,4, 6, 8 weeks after operations respectively. The rats,choosed from successful models, were poured and fixed,then floating slices, frozen slices and vibration slices of the brain were made separately 2weeks,1 month and 2 months after operation. The specimen were examined by ABC and revealed that the Tyrosine hydroxylase (TH) positive cells were decreased to about 15% in surviving 2 weeks rats. About 98% TH positive cells of compact substantia nigra were disappeared in surviving 1 month and 2 months rats.Right substantia nigra cells were found to be reduced to 5% with massive proliferation of glias by TH and nissl double technique, while the left substantia nigra areas were remain normal.This study confirms that dopaminergic and noradrenaline neurons were selectively destroyed by 6-OHDA.
出处
《上海实验动物科学》
1995年第1期2-5,共4页
Shanghai Laboratory Animal Science
关键词
大鼠
震颤性麻痹
动物模型
hydroxydopamine(6-OHDA)
Apomorphine
Tyrosine hydroxylase
Substantia nigra