^（131）Ⅰ－McAhJHⅢ对裸鼠荷人肝细胞癌的放射免疫显像研究

Radioimmunoimaging of ￣(131)Ⅰ-Labeled MonoclonalAntibody JHⅢ in Nude Mice Bearing HumanHepatocellular Carcinoma

用氯胺Ｔ法标记抗人肝癌单克隆抗体（ＭＣＡｂＪＨⅢ），对荷人肝细胞癌ＢＥＬ－７４０２裸鼠进行体内生物学分布和肿瘤放射免疫显像研究。腹腔注射１３１Ⅰ－ＭＣＡｂＪＨⅢ后２４～１２０ｈ，肿瘤部位的放射性浓聚，以７２～９６ｈ的影像最为清晰；而注射１３１Ⅰ－ｍＩｇＧ则呈全身性均匀分布，无肿瘤部位选择性浓聚现象。１３１Ⅰ－ＭｃＡｂＪＨⅢ注射后７２ｈ，１３种器官的肿瘤组织与非瘤组织的放射性比值均大于５，肿瘤的定位指数为７．０７。这表明ＭＣＡｂＪＨⅢ在肝细胞癌的诊断和导向治疗方面可能有较好的应用价值。

he monoclonal antibody JHⅢ (McAbJHⅢ ) against human hepatocellular carcinoma was radiolabeled with 131I by Ch-T method. Its distribution and radioimmnunoimaging in the nude mice bearing human hepatoma BEL-7402 were studied. Between 24-120 h after intraperitoneal administration, the image of the concentrating radioactivity in tumor was obtained by the y camera and it was the clearest during 72-96 h;whereas the injection of 131I-mIgG into the nude mice resulted in an equal distribution in the whole body without any selectivity. At 72 hafter the injection of 131I-McAb JHⅢ, the radioactivity ratioes of tumor/normal tissue (T/NT) for 13 kinds of organ/tissue were all over 5, and the localization index of tumor was 7.07. These results showed that McAbJHⅢ may be used in the localization and targeting treatment of human hepatocellular carcinoma.