ELISA竞争抑制法检测实验动物的多杀性巴氏杆菌

Detection of Pasteurella Multocida by ELISA Inhibitory Test in Laboratory Animals

设计了一种灵敏的ＥＭＩＳＡ竟争抑制法，用以检测实验动物多杀性巴氏杆菌。在该系统中，从多杀性巴氏杆菌Ｐ－１０５９、Ｘ－７３和Ａ－８９８中抽提可溶性物质作为包板抗原。用３株全菌体免疫兔，提纯兔抗多杀性巴氏杆菌ＩｇＧ，用辣根过氧化物酶标记兔抗多杀性巴氏杆菌ＩｇＧ，将酶结合物与待检动物血清做ＥＬＩＳＡ竞争抑制试验。同时用常规ＥＬＩＳＡ间接法做验证实验。结果表明此法准确可靠，可用于各种实验动物的多杀性巴氏杆菌的检测。对小鼠、仓鼠、兔的检测表明，兔的Ｐ－１０５９阳性率达３０％，小鼠、仓鼠未见阳性。

A rapid sensitive ELISA inhibitory test was designed to detect Pasteurella multocida infection in laboratory animals. In the reactive system. the soluble materialextracted from P. multocida(p.m)strain P-1059, X-73 or A-898 was used as coatingantigen. rabbits were immunized with these three strains of whole bacteri resapectively. The purified rabbits IgG of anti-p.m was labeled with HRP by sodium periodatemethod. The HRP labeled rabbit anti-p. m IgG was mixed with animal serum samples to operate ELISA inhibitory test. The conventional indirect ELISA was performed to confirm the results. It was found that infective rate of P. m strain P-1059in rabbits was as high as 30%; while in mice and bamsters, no positive sampleswere detected. The results indicated that this is an easy, rapid and reliable methodfor the detection of P. m infection in laboratory animals.