摘要
整体红细胞(RBQ)可促进自然杀伤细胞(NK)的活性,在一定浓度范围内其提高NK细胞活性的程度与RBC数量呈正比,低渗破膜分离胞膜和胞质成份后,发现胞质液具有明显的促进NK活性作用,而胞膜却无促进作用,胞质液促进NK活性的作用方式与IFN-r,IL-2或其它一些微量元素不同,即预孵育效应细胞或靶细胞不能提高NK活性,而必需有效应细胞靶细胞胞质液三者同时存在时方表现出来,以上结果提示整体红细胞促进NK活性作用机制可能与胞质液成份有关。
NK ce11- mediated cytotoxicity can be augmented by red b1ood cell(RBC).Theenhancement is dose dependent on the number of RBC. After RBC is lysed with distilled deionized water,cytosol and membrane fractions are obtained by centrifugation. It isfound that cytosol can also enhance NK activity greatly and the enhancement is observedonly when cytosol is present during the cytotoxicity assay. These suggest that the enhan-cemenr of NK activity induced by RBC is probably through releasing cytosol.
出处
《上海医学》
CAS
CSCD
北大核心
1995年第1期5-7,共3页
Shanghai Medical Journal
关键词
细胞毒
活性
红细胞
胞质液
杀伤细胞
NK cell-mediated cytotoxicity,Red blood cell ,Cytosol