红细胞促进NK细胞免疫活性的研究

STUDY ON MODULATION OF NK CELL-MEDIATED CYTOTOXICITY BY RED BLOOL CELL AND ITS SUBCELLULAR COMLPONENTS

整体红细胞（ＲＢＱ）可促进自然杀伤细胞（ＮＫ）的活性，在一定浓度范围内其提高ＮＫ细胞活性的程度与ＲＢＣ数量呈正比，低渗破膜分离胞膜和胞质成份后，发现胞质液具有明显的促进ＮＫ活性作用，而胞膜却无促进作用，胞质液促进ＮＫ活性的作用方式与ＩＦＮ－ｒ，ＩＬ－２或其它一些微量元素不同，即预孵育效应细胞或靶细胞不能提高ＮＫ活性，而必需有效应细胞靶细胞胞质液三者同时存在时方表现出来，以上结果提示整体红细胞促进ＮＫ活性作用机制可能与胞质液成份有关。

NK ce11- mediated cytotoxicity can be augmented by red b1ood cell(RBC).Theenhancement is dose dependent on the number of RBC. After RBC is lysed with distilled deionized water,cytosol and membrane fractions are obtained by centrifugation. It isfound that cytosol can also enhance NK activity greatly and the enhancement is observedonly when cytosol is present during the cytotoxicity assay. These suggest that the enhan-cemenr of NK activity induced by RBC is probably through releasing cytosol.