用瞬时甲基化气液色谱法测定脑组织中游离脂肪酸

DETERMINATION OF BRAIN TISSUE FREE FATTY ACIDS BY FLASH METHYLATIVE GASLIQ UID CHROMATOGRAPHY

用瞬时甲基化气液色谱法（ＧＬＣ）分离和测定脑组织中存在游离脂肪酸（ＦＦＡ，包括棕榈酸Ｃ１６：０、油酸Ｃ１８：１、硬脂酸Ｃ１８：０、花生四烯酸Ｃ２０：４、廿二碳六烯酸Ｃ２２：６）。最低检测限为０．０２８～０．０４５ｎｍｏｌ，用十五烷酸Ｃ１５：０作内标，测得５种标准脂肪酸的线性范围为０．１１～１．７４ｎｍｏｌ，相关系数ｒ＝０．９９９２～０．９９９９，方法回收率为９５．３６％～９８．７７％，天内精密度ＲＳＤ＜５．６０％；天间精密度ＲＳＤ＜５．７９％。

A convenient and reliable flash methylative gas-liquid chromatographic methed for determining free fatty acids(FFA)in brain tissue specimens was studied.The limit of detection of FFA was 0.028～0.045 nmol.With pentadecanoic acid(C15:0)as an internal standard,a linear relationship was from 0.11～1.74 nmol(five FFA,r=0.999 2～0.9999).The overall percentage recovery of FFA was 95.36%～98.77%.Their RSD were<5.60% within day,and<5.79% between days.