胚腹侧中脑移植入震颤麻痹症模型鼠靶区与非靶区的实验研究

AN EXPERIMENTAL STUDY ON FETAL VENTRAL MESENCEPHALON TRANSPLANTS INTO TARGET AND NONTARGET AREAS IN RAT MODEL OF PARKINSON'S DISEASE

用６－羟基多巴胺注入ＳＤ鼠右侧被盖腹侧区黑质内侧端，制成震颤麻痹症动物模型．毁损后３～５周，用胚龄１５ｄ的胚鼠腹侧中脑混悬液植入模型鼠靶区──纹状体与非靶区──黑质、丘脑等部位．动物存活８～１２周后处死，于尾壳核、黑质及丘脑均可见有存活的移植区．移植区内均可见ＴＨ免疫阳性细胞．本实验表明，用胚腹侧中脑移植入震颤麻痹症模型鼠靶区及非靶区，移植物均能存活、生长并发挥其功能效应．

Patkinson's disease (PD) rat model was produced by injection of 6-OHDA into the right medial part of substantia nigra. 3～4 weeks post lesion, fetal (E15) dopaminergic cell suspension was implanted into the target area-striatum and nontarget area-substantia nigra and thalamus. 2～3 months after grafting, the survival grafts were seen either in the host striatum, or in the substantia nigrp and thalamus. TH positive neurons were found in the target and nontarget graft areas and the rotation tests were improved in these groups. The results indicated that the fetal ventral mesencepbalon neurons implanted into target and nontarget areas of PD model rats, not only can survive and grow well but also ameliorate the behavioural deficits.