化学培养液对小鼠原核胚体外发育的影响

Effect of Chemical Media on in Vitro Development of Mouse Pronuclear Embryos

本试验用０．１ｍＬ培养液在３７℃，５％ＣＯ２条件下培养昆明小鼠原核胚。结果表明，用ｍＳＯＦ和ＰＬ３培养小鼠原核胚，４－细胞发育率分别为１０％和７７％，以后停止发育；用ＰＬ３和ＰＬ３’培养小鼠原核胚，每２４ｈ换１次培养液，结果发育到桑椹胚和囊胚的百分率分别是２７．５％和３８．５％；用ｍＭＴＦ，ｍＭＴＦ＋２０ＡＡ，ｍＭＴＦ＋Ｎｏｎ＋Ｇｌｎ，ｍＭＴＦ＋Ｎｏｎ，ｍＭＴＦ＋Ｅｓｓ，ｍＭＴＦ＋Ｅｓｓ＋Ｇｌｎ培养小鼠原核胚，发育率分别为５７．１％，５１．０％，５６．７％，５８．１％，５９．１％和５４．９％，各组间差异不显著（Ｐ＞０．０５）。试验证明，几种培养液均可使小鼠原核胚克服阻断，继续发育，但改换培养液有利于其进一步发育；氨基酸对小鼠胚胎发育有促进作用．

The result obtained from chemical media at 37℃ in 5% CO2 in air on in vitro development of Kunming mouse pronuclear embryos were that the developmental rates were 15% and 77%, resp., with significant difference (P>0.001)during cultured with mSOF and PL3; the developmental rates were 27.5% and 38.5%,resp. when cultured in PL3 and PL3 and replacing with fresh media one time per 24h;the mouse pronuclear embryos cultured in mMTF+20 AA, mMTF+Non+Gln,mMTF+Non, mMTF+Ess and mMTF+Ess+Gln got the developmental rates of 57.1%, 51.0%,56.7%, 58.1%, 59.1% and 54.9%, resp., with no significant difference (P>0.05). The above results demonstrate that chemical media can support in the development of mousepronuclear embryos.Amino acids can play a role on mouse embryonic development.