柴胡皂甙d对实验性膜性肾炎疗效及作用机理研究

Studies on the effects of saikosaponin d on experi-mental membranous nephropathy in rats and itsmechanisms

本文观察了从中药柴胡中提取的单体成分柴胡皂甙ｄ（ＳＳｄ）对被动性Ｈｅｙｍａｎｎ肾炎（ＰＨＮ）的疗效。结果表明，用ＳＳｄ治疗可以减轻ＰＨＮ大鼠蛋白尿，肾小球病理变化也得到不同程度的改善。血清皮质酮水平和肾皮质超氧化物歧化酶（ＳＯＤ）活性升高，血清甘油三酯含量和肾皮质磷脂酶Ａ＿２（ＰＬＡ＿２）活性降低，肾小球内ＩｇＧ和Ｃ３的沉积明显减少。提示ＳＳｄ的抗肾炎作用与其促进内源性糖皮质激素分泌、抑制ＰＬＡ＿２活性、阻止ＩｇＧ和Ｃ３在肾小球内沉积、提高ＳＯＤ活性及改善异常的脂质代谢等作用密切相关。

he effects of saikosaponin d (SSd) extractedfrom Bupleurum falcatum L.on passive Heymannnephritis (PHN) in rats and its mechanisms werestudied in this paper.The results obtained were asfollows :① After SSd were given (0. 1mg/100g. d )for three weeks by intraperitoneally injection (i. p),urine protein was lowered significantly, whileglomerular pathological changes were inhibited to agreater or lesser degree in rats with PHN ;②AfterSSd being given (0. 1mg/100g.d,ip ) for threeweeks,increased serum corticosterone level and re-nal cortex superoxide dismutase(SOD) activity ,de-creased renal cortex phospholipase A_2 (PLA_2) activ-ity,lowered serum triglyceride level were observed ,and deposits of rabbit IgG, rat IgG and C3 inglomeruli were also decreased. These changes weresignificant and suggested that antinephritic mecha-nisms of SSd were due to its anti-inflammatory ac-tion by enhancing the release of adrenal glucocorti-coid and inhibiting PLA_2 activity, immune-sup-pressing action by preventing IgG and C3 from de-positing in glomeruli, and actions of enhancing theactivity of reactive oxygen scavenger SOD and im-proving abnormal lipid metabolism.